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TRANSCRIPTOMICS 30 days · 6 modules · Online

miRNA-Seq Data Analysis Workshop (30-Day, Online)

Process small-RNA data, discover known and novel miRNAs and predict their downstream target genes.

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About this workshop

MicroRNAs are very short RNA molecules, around 22 nucleotides, that switch off genes after transcription. Because the reads are so short, small-RNA sequencing needs its own handling, from trimming adapters to keeping only reads in the right size range. This workshop covers that pre-processing and then moves to identifying known miRNAs, spotting sequence variants of the same miRNA and predicting new candidates. You compare expression between groups, predict which genes each miRNA targets, and combine miRNA and mRNA results to find pairs that move in opposite directions. The workshop ends with pathway interpretation and regulatory networks that show what the changes may mean. It suits researchers studying gene regulation in disease, development or stress.

Tools and pipelines you will use

This workshop emphasizes standard, open-source bioinformatics tools widely used in peer-reviewed genomics literature:

What you will learn

Module 1 Foundations
Week 1
Linux and command-line basics Run tools and organise data.
miRNA biology Biogenesis and how miRNAs silence genes.
Small-RNA library design Sizes, adapters and common pitfalls.
Read quality control Check quality in short reads.
Module 2 Preprocessing
Adapter removal Trim adapters from very short reads.
Length filtering Keep reads in the miRNA size range.
Collapsing reads Count identical sequences efficiently.
Preprocessing report Summarise how many reads survive each step.
Module 3 Identification and quantification
Mapping to miRNA databases Match reads to known miRNAs.
Novel miRNA discovery Predict new candidates from genome mapping.
Isoforms (isomiRs) Detect sequence variants of the same miRNA.
Counting and normalization Prepare counts for comparison.
Module 4 Differential expression
Sample-level checks Use PCA and clustering to inspect data.
Statistical testing Find miRNAs that change between groups.
Visualizing results Volcano plots and heatmaps.
Choosing thresholds Decide what counts as meaningful change.
Module 5 Target prediction and interpretation
Target prediction tools Predict genes regulated by each miRNA.
Combining miRNA and mRNA data Find inversely changed pairs.
Pathway enrichment Interpret targets biologically.
Regulatory networks Draw miRNA-gene networks.
Module 6 Mini-project and report
Week 4
Project dataset Analyse a public small-RNA dataset.
Reproducible workflow Record every step and setting.
Figures and write-up Present key miRNAs and targets.
Review and next steps Discuss validation options.

What you receive & prerequisites

What you receive

  • 30 days of structured online self-paced access & mentor guidance
  • Curated real-world NGS benchmark datasets
  • Reusable pipeline scripts and command-line code templates
  • Independent capstone mini-project evaluation
  • Certificate of Completion issued by Xenoi Biotech Pvt. Ltd.

Prerequisites & setup

  • Basic life science background (B.Sc., M.Sc., B.Tech, or research scholar)
  • No prior programming or Linux experience required (covered in Module 1)
  • Laptop or PC with 8 GB RAM and internet access
  • Remote compute setup instructions provided during onboarding

Course curriculum designed by Anjali Chaudhary, Bioinformatics Scientist at Xenoi Biotech.

Last updated: October 2026

Frequently asked questions

Is this miRNA-Seq workshop delivered online?

Yes. All Xenoi Biotech workshops are delivered 100% online, allowing researchers and students across India and internationally to participate with remote compute support.

How long is the workshop and what is the workload?

The workshop runs for 30 days across 6 structured modules. Learners typically dedicate 1 to 2 flexible hours per day to work through pipeline commands, code exercises, and the mini-project.

Who is this workshop designed for?

It is built for B.Sc., M.Sc., and Ph.D. students, postdoctoral researchers, faculty members, and industry professionals in biotechnology, biochemistry, microbiology, and medicine who need to analyze NGS datasets independently.

What will I learn in this workshop?

You will learn small-RNA adapter trimming, miRDeep2 quantification and novel miRNA prediction, differential expression in DESeq2, miRNA-target prediction with TargetScan and miRDB, and integrated miRNA-mRNA network construction.

What are the fees, dates, and enrollment details?

The workshop fee is MRP ₹5,000, inclusive of GST. Batch dates are shared on enquiry. Use the Enrol now button to reserve your place or receive the upcoming schedule.

Fees, dates and batches

The workshop fee is MRP ₹5,000 (incl. GST). Batch dates and onboarding materials are shared upon enquiry.

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