16S & ITS Metagenomics Data Analysis Workshop (30-Day, Online)
Process amplicon sequencing data, assign exact taxonomy and compare microbial diversity across environments.
Next batch
- Duration: 30 days, 6 modules, online
- Fee: MRP ₹5,000 (incl. GST)
About this workshop
Targeted metagenomics identifies the microbes in a sample by sequencing one marker gene, the 16S rRNA gene for bacteria and archaea or the ITS region for fungi. It is a common first step in microbiome studies of gut, soil, water and plant samples. This workshop shows how to turn amplicon reads into results: removing primers, denoising reads into exact sequence variants, removing chimeras and assigning taxonomy against a reference database. You then describe diversity within and between samples, make ordination plots and test which taxa differ between groups, with attention to the pitfalls of compositional data and unequal sequencing depth. You also learn to predict likely community functions from the marker data and to present the results clearly.
Our research: Explore our team's published microbiome investigations: Tina Roy et al. (2025), Advancement in rhizospheric microbial diversity analysis: an updated perspective (DOI: 10.1007/s13562-025-01013-3) and Tina Roy et al. (2024), Rhizosphere Microbiome-Assisted Approaches for Biotic Stress Management (DOI: 10.1007/978-981-97-4239-4_8).
Tools and pipelines you will use
This workshop emphasizes standard, open-source bioinformatics tools widely used in peer-reviewed genomics literature:
What you will learn
Module 1
Foundations
Week 1
Module 2
Amplicon processing
Module 3
Taxonomy
Module 4
Diversity
Module 5
Differential abundance and function
Module 6
Mini-project and report
Week 4
What you receive & prerequisites
What you receive
- 30 days of structured online self-paced access & mentor guidance
- Curated real-world NGS benchmark datasets
- Reusable pipeline scripts and command-line code templates
- Independent capstone mini-project evaluation
- Certificate of Completion issued by Xenoi Biotech Pvt. Ltd.
Prerequisites & setup
- Basic life science background (B.Sc., M.Sc., B.Tech, or research scholar)
- No prior programming or Linux experience required (covered in Module 1)
- Laptop or PC with 8 GB RAM and internet access
- Remote compute setup instructions provided during onboarding
Frequently asked questions
Is this Targeted Metagenomics (16S / ITS) workshop delivered online?
Yes. All Xenoi Biotech workshops are delivered 100% online, allowing researchers and students across India and internationally to participate with remote compute support.
How long is the workshop and what is the workload?
The workshop runs for 30 days across 6 structured modules. Learners typically dedicate 1 to 2 flexible hours per day to work through pipeline commands, code exercises, and the mini-project.
Who is this workshop designed for?
It is built for B.Sc., M.Sc., and Ph.D. students, postdoctoral researchers, faculty members, and industry professionals in biotechnology, biochemistry, microbiology, and medicine who need to analyze NGS datasets independently.
What will I learn in this workshop?
You will learn demultiplexing, primer trimming, DADA2 amplicon sequence variant (ASV) inference, taxonomic classification against SILVA/Greengenes, alpha and beta diversity metrics, PCoA ordination plots, and functional metagenome prediction with PICRUSt2.
What are the fees, dates, and enrollment details?
The workshop fee is MRP ₹5,000, inclusive of GST. Batch dates are shared on enquiry. Use the Enrol now button to reserve your place or receive the upcoming schedule.
Fees, dates and batches
The workshop fee is MRP ₹5,000 (incl. GST). Batch dates and onboarding materials are shared upon enquiry.
Enquire about this workshop ↗